Effects of Methanolic Leaves Extract Of Balanites Aegyptiaca Del on Some Biochemical Parameters in Wistar Strain Albino Rats

Attah Jacob, Binta Baba Shehu, Attah Martha Orendu


Background: Balanites Aegyptiaca leaves has been used as ingredient and in the preparation of local foods and serves as a medicinal plant in many developing countries. The objective is to elucidate the effect of methanolic leave extract of Balanites Aegyptiaca on some biochemical parameters in Wistar rats.

Methods: A three week repeated dose toxicity study of methanolic extract of Balanites Aegyptiaca  leaves was performed on Wister strain rats separated into four groups of five animals each. Group 1 the control group, Group 2, 3 and 4 administered 200mg/kg, 400mg/kg and 800mg/kg of the extract respectively. They were sacrificed after 21 days and blood collected for determination of biochemical parameters.

Results: The results shows a significant decrease (P<0.05) in the level of alkaline phosphatase but no significant change in the levels of ALAT, ASAT, total protein, albumin, creatinine, urea, sodium, potassium and chloride.

Conclusion: Administration of the methanolic leave extract of Balanites Aegyptiaca  did not reveal marked changes in the in the toxicological parameters been assayed except for the observed decrease in alkaline phosphatase.

 Keywords: Alanine Transaminase , Aspartate Transaminase, Balanites Aegyptiaca , Body Weight, Wistar Rats, Elderly, Cerebrovascular diseases.

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Barley S., Watt J. M. and Breyer-Brandwijk M. GThe Medicinal and poisonous plants of Southern and Eastern Africa. London: Livingstone Ltd. (1962). p. 1064.

Chawla R. Practical Clinical Biochemistry (methods and interpretations). Second edition. Jaypee Brothers Medical Publishers, New Delhi, India.1999 pp 106-108 ISBN: 9350909421

Doughari J. M. and Pukuma M. S. Antibacterial effects of Balanites Aegyptiaca L. Del. and Moringaoleifera Lam. on Salmonella typhi. African Journal of Biotechnology. 20076:2212–2215.

Dunnett C. W. A multiple comparison procedure for comparing several treatments with control. Fournal of American science association. 1955 12:1096-1121

Khan F. M. Ethno-veterinary medicinal usage of flora of greater cholistan desert (Pakistan) Pakistan Vetenary Journal. 2009 29:75–80.

Kamel M. S., Ohtani K., Kurokawa T., Assaf M. H., El-Shanawany M. A. and Ali A. A. Studies on Balanites Aegyptiaca fruits, an antidiabetic Egyptian folk medicine. Chemical Pharmacological journal. 1991 39:1229–1233. PMID: 1913998

Kamel M. S. A furostanolsaponin from fruits of Balanites Aegyptiaca. African journal of Phytochemistry. 1998 48:755–757. nopr.niscair.res.in/bitstream

Moriyama T., Miyazawa H., Tomohiro M. and Nishikibe M. Beneficial effect of moderate food restriction in toxicity studies in rats. Journal of toxicological science. 2006 31(3): 197-206. PMID: 16960430

Obidah W., Nadro M. S., Tiyafo G. O. and Wurochekke A. U. Toxicity of crude Balanites Aegyptiaca seed oil in rats. Journal of American Science. 2009 5:13–16.

Ojo O. O., Nadro M. S. and Tella I. O. Protection of rats by extracts of some common Nigerian trees against acetaminophen-induced hepatotoxicity. African Journal of Biotechnology. 2006 5:755–760.

Oliver-Bever . Medicinal plants in tropical West Africa. Cambridge: Cambridge University Press; 1986 pp. 54, 184–185.

Samuelsson G., Farah M. H. and Claeson P. Inventory of plants used in traditional medicine of Somalia, plant of the families Acanthaceae- Chenopodiaceae. Journal 0f Ethanopharmacology.1991 35:25–63. PMID: 1753795

M. S. Nadro and F. P. Samson, The effects of Balanite Aegyptiaca kernel cake as supplement on alloxan-induced diabetes mellitus in rats, Journal of Applied Pharmaceutical Science Vol. 4 (10), pp. 058-061, October, 2014, DOI: 10.7324/JAPS.2014.40111


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DOI Prefix: 10.20286