Ethno-Pharmacological Survey, In Vitro Anti-Sickling and Free Radical Scavenging Activities of Carapa Procera DC. Stem Bark (Meliaceae)

Koto-te-Nyiwa Ngbolua, Tshitenge T. Bishola, Tshimankinda P. Mpiana, Virima Mudogo, Sha-Tshibey D. Tshibangu, Kabamba N. Ngombe, Elumba G. Ekutsu, Zoawe B. Gbolo, Ngandu O. Kabena

Abstract


Drepanocytosis is a genetic and neglected disease, endemic in negroids population. One of the main characteristics of this pathology is the production of a large amount of free radicals, leading to a severe oxidative stress and the consumption of NO by free oxygen radicals, and/or by cell-free plasma heme. The consequences of this defect are hemolytic anemia and tissue damage brought about by the blockage of blood vessels by the sickled cells. The present study evaluated the antisickling and radical scavenging activities of extracts from Carapa procera stem bark using Emmel’s test and the DPPH assay. Carapa procera was selected through an ethno-pharmacological. The results showed that methanolic, ethyl acetate and dichloromethane soluble fractions, anthocyanins and organic acids exhibited a significant antisickling as revealed by the observed normal biconcave form of sickle erythrocyte (normalization rate > 70%) in hypoxic conditions. Methanolic extract exhibited a good radical scavenging activity (ED50 = 1.698 ± 0.079 µg/mL). The chemical screening performed on the plant revealed the presence of anthocyanins and organic acids which were then extracted. Total anthocyanins and organic acids revealed interesting antisickling and antioxidant properties that could justify the integration of Carapa procera in Congolese pharmacopoeia for the management of sickle cell disease. Bioactive extracts from this plant species could increase nitric oxide by scavenging free oxygen radicals. For the best our knowledge, Carapa procera has not been yet previously reported as antisickling plant in the traditional medicine database of Democratic Republic of the Congo. 


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Mpiana PT, Ngbolua K, Mudogo V, Tshibangu D, Atibu E, Mbala B, et al. The potential effectiveness of medicinal plants used for the treatment of sickle cell disease in the Democratic Republic of Congo folk medicine: A review. Progress in Traditional and Folk herbal medicine. 2012;1:1-11.

Organization WH. Drépanocytose et autres hémoglobinopathies. 2012.

Mpiana P, Tshibangu D, Shetonde O, Ngbolua K. In vitro antidrepanocytary actvity (anti-sickle cell anemia) of some congolese plants. Phytomedicine. 2007;14:192-5.

Mpiana P, Mudogo V, Tshibangu D, Kitwa E, Kanangila A, Lumbu J, et al. Antisickling activity of anthocyanins from Bombax pentadrum, Ficus capensis and Ziziphus mucronata: photodegradation effect. Journal of ethnopharmacology. 2008;120:413-8.

Mpiana P, Mudogo V, Tshibangu D, Ngbolua K, Shetonde O, Mangwala P, et al. In vitro antisickling activity of anthocyanins extracts of a Congolese plant: Alchornea cordifolia M. Arg. Journal of Medical Sciences. 2007;7:1182-6.

Mpiana P, Mudogo V, Ngbolua K, Tshibangu D, Shetonde O, Mbala M. In vitro antisickling activity of anthocyanins from Ocimum basilicum L.(Lamiaceae). 2007.

Mpiana P, Mudogo V, Kabangu Y, Tshibangu D, Ngbolua K, Atibu E, et al. Antisickling activity and thermostability of anthocyanins extract from a congolese plant, Hymenocardia acida Tul.(Hymenocardiaceae). IJP-International Journal of Pharmacology. 2009;5:65-70.

Kambale J, Ngolua K, Mpiana P, Mudogo V, Tshibangu D, Wumba D, et al. Evaluation in vitro de l’activité antifalcémiante et effet antioxydant des extraits d’Uapaca heudelotii Baill.(Euphorbiaceae). International Journal of Biological and Chemical Sciences. 2013;7:523-34.

Ngbolua K, Fatiany P, Robijaona B, Randrianirina A, Rajaonarivelo P, Rasondratovo B, et al. Ethno-botanical survey, Chemical composition and in vitro Antimicrobial activity of essential oils from the root bark of Hazomalania voyroni (Jum.) Capuron (Hernandiaceae). Journal of Advancement in Medical and Life Sciences. 2014;1:01-6.

Ngbolua K, Mpiana P, Mudogo V, Ngombe N, Tshibangu D, Ekutsu E, et al. Ethno-pharmacological survey and Floristical study of some Medicinal Plants traditionally used to treat infectious and parasitic pathologies in the Democratic Republic of Congo. International Journal of Medicinal Plants. 2014;106:454-67.

Kasali F, Mahano A, Nyakabwa D, Kadima N, Misakabu F, Tshibangu D, et al. Ethnopharmacological survey of medicinal plants used against malaria in Bukavu city (DR Congo). European Journal of Medicinal Plants. 2014;4:29.

Tshilanda DD, Mpiana PT, Onyamboko DNV, Mbala BM, Tshibangu DST, Bokolo MK, et al. Antisickling activity of butyl stearate isolated from Ocimum basilicum (Lamiaceae). Asian Pacific journal of tropical biomedicine. 2014;4:393-8.

Mpiana P, Misakabu F, Yuma P, Tshibangu D, Ngbolua K, Mwanyishay C, et al. Antisickling activity and physico-chemical stability of anthocyanin extracts from Ipomoea Batatas leaves. infection. 2014;6:8.

Yuma P, Mpiana P, Bokota M, Wakenge I, Muanishay C, Gbolo B, et al. Étude de l’activité antifalcemiante et de la thermo-et photo-dégradation des anthocyanes de Centella asiatica, Thomandersia hensii et Maesopsis eminii. International Journal of Biological and Chemical Sciences. 2014;7:1892-901.

Ngbolua K, Mudogo V, Mpiana P, Malekani M, Rafatro H, Ratsimamanga U, et al. Evaluation de l’activité anti-drépanocytaire et antipaludique de quelques taxons végétaux de la République démocratique du Congo et de Madagascar. Ethnopharmacologia. 2013;50:19-24.

Mpiana PT, Lombe BK, Ombeni AM, Tshibangu DS, Wimba LK, Tshilanda DD, et al. In Vitro Sickling Inhibitory Effects and Anti-Sickle Erythrocytes Hemolysis of Dicliptera colorata CB Clarke, Euphorbia hirta L. and Sorghum bicolor (L.) Moench. 2013.

Mpiana P, Dianzenza E, Ngbolua K, Tshibangu D, Mbala B, Mhigo S, et al. Antisickling properties, thermal and photochemical degradations of anthocyanin extracts from Annona senegalensis (Annonaceae). International Journal of Biological and Chemical Sciences. 2013;6:2241-51.

Mpiana P. Inhibitory Effect of Anthocyanins Extracts of T rema orientalis. J Med Sci. 2011;11:129-37.

Mpiana PT, Ngbolua KNN, Bokota MT, Kasonga TK, Atibu EK, Tshibangu DS, et al. In vitro effects of anthocyanin extracts from Justicia secunda Vahl on the solubility of haemoglobin S and membrane stability of sickle erythrocytes. Blood transfusion. 2010;8:248.

Mpiana P, Bokota M, Mbula J, Ngbolua K, Tshibangu D, Atibu E, et al. Effet of Anthocyanins Extracts from Justicia matammensis and Justicia laxa on Sickle cells. Anthocyanins: structure, biosynthesis and health benefits New York: Nova publishers. 2012:111-24.

Kähkönen MP, Heinonen M. Antioxidant activity of anthocyanins and their aglycons. Journal of agricultural and food chemistry. 2003;51:628-33.

Satué-Gracia MT, Heinonen M, Frankel EN. Anthocyanins as antioxidants on human low-density lipoprotein and lecithin-liposome systems. Journal of Agricultural and Food Chemistry. 1997;45:3362-7.

Tshibangu D, Shode F, Koorbanally N, Mudogo V, Mpiana P, Ngbolua K, editors. Antisickling triterpenoids from Callistemon viminalis, Meulaleuca bracteata var. Revolution Gold Syzygium guineense and Syzygium cordatum. The 14th NAPRECA Symposium and AAMPS Ethno-veterinary Medicine Symposium 8th-12th August; 2011.

Ngbolua K, Benamambote B, Mpiana P, Muanda D, Ekutsu E, Tshibangu D, et al. Ethno-botanical survey and Ecological Study of some Medicinal Plants species traditionally used in the District of Bas-Fleuve (Bas-Congo Province, Democratic Republic of Congo). Research Journal of Chemistry. 2013;1:01-10.

Coombe R. Protecting traditional environmental knowledge and new social movements in the Americas: intellectual property, human right or claims to an alternative form of sustainable development? Human Right or Claims to an Alternative Form of Sustainable Development. 2005:115-35.

Buck M, Hamilton C. The Nagoya Protocol on access to genetic resources and the fair and equitable sharing of benefits arising from their utilization to the Convention on Biological Diversity. Review of European Community & International Environmental Law. 2011;20:47-61.

Soares J. The Nagoya Protocol and natural product-based research. ACS chemical biology. 2011;6:289-.

Bruneton J. Pharmacognosie: phytochimie plantes médicinales. 1993.

Ouattara B, Angenot L, Guissou P, Fondu P, Dubois J, Frédérich M, et al. LC/MS/NMR analysis of isomeric divanilloylquinic acids from the root bark of Fagara zanthoxyloides Lam. Phytochemistry. 2004;65:1145-51.

Scherer R, Godoy HT. Antioxidant activity index (AAI) by the 2, 2-diphenyl-1-picrylhydrazyl method. Food Chemistry. 2009;112:654-8.

Mpiana P, Makelele L, Oleko R, Bokota M, Tshibangu D, Ngbolua K, et al. Antisickling activity of medicinal plants used in the management of sickle cell disease in the Tshopo district, DR Congo. Australian Journal of Medical Herbalism. 2010;22:132.

Dasgupta T, Hebbel RP, Kaul DK. Protective effect of arginine on oxidative stress in transgenic sickle mouse models. Free Radical Biology and Medicine. 2006;41:1771-80.




DOI: http://dx.doi.org/10.20286/nova-jmbs-030130

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DOI Prefix: 10.20286