Assessment of Protein Content and Study Effect of Anti-Nutrition Factor on in-vitro Protein Digestibility

MK Sabahelkhier, SIY Adam, MOS Rayan, IAA Amana, MA Maab, AAI Nusiba

Abstract


Aims of this study were assessed the protein content of dry meat, raw egg, dehulled seeds of wheat, pigeon pea and study effect of concentration 1, 2 and 3% of the Pure Tannin, Solenostemma argel, Artemisia herba alba, Process green tea and Moringa oleifera on in-vitro protein digestability of animal and plant protein. Experiment: It was carried out in lab of Department of Food Science and Technology, Faculty of Agriculture, University of Khartoum in 2014. Methodology: Micro-Kjeldhal method and in-vitro protein digestability were used. Results: Results indicated that protein content of dry meat (77%) and raw egg (79%) was higher than protein content of dehulled seeds of wheat (18%) and dehulled seeds of pigeon pea (22%). There was significant difference between the four samples in protein at P ≤ 0.05. In-vitro protein digestability of dry meat, raw egg, dehulled seeds of wheat and pigeon was clearly reduced with increasing the concentration of pure tannin. While in-vitro protein digestability for dehulled wheat was clearly increased with increasing the concentration of S. argel leaves, but no significant was observed in dehulled seeds of pigeon pea, but at concentration 3% S. argel, the in-vitro protein digestability was decreased. In case of dry meat and raw egg in-vitro protein digestability was reduced with adding 1, 2 and 3% S. argel leaves. Whereas, in-vitro protein digestability for dehulled seeds of wheat was improved with increasing concentration of S. argel, but in-vitro protein of dehulled pigeon pea was decreased with increasing concentration of S. argel. While in-vitro protein digestability for dry meat, raw egg, dehulled seeds of wheat and pigeon pea was reduced with increasing concentration of A. herba alba, process green tea and M. oleifera, but at concentration 3% of moringa the in-vitro protein digestability of dehulled seeds of pigeon pea (75%) was improved. Conclusion: Protein content is very rich in animal products, but it is very poor in plant products. Uses of S. argel with dehulled seeds of wheat was improved its in-vitro protein digestability, but uses of pure tannic acid, A. herba alba and green tea reduced in-vitro protein digestability.


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References


Williams P. Nutritional composition of red meat. Nutr Diet. 2007;64:S113-S9.

Watkins B. The nutritive value of the egg. In: Stadelman W, Cotterill O, editors. Egg Science and Technology. New York: The Haworth Press Inc; 1995. p. 177- 94.

Khalil S. An assessment of the Wheat Crop. PARC, Pakistan, Antinutritional factors. In: Liener I, editor. Chemistry, Technology andHuman Nutrition (ed RH Mathews) New York and Basel: Marcel Dekker, Inc.; 2006. p. 339–82.

Sosulski F, Humbert E, Bui K, Jones J. Functional Propreties of Rapeseed Flours, Concentrates and Isolate. J Food Sci. 1976;41:1349-52.

Moyo B, Masika PJ, Hugo A, Muchenje V. Nutritional characterization of Moringa (Moringa oleifera Lam.) leaves. Africa J Biotech. 2013;10:12925-33.

Roberts G, Sanderson G. Tea Chemistry. J Sci Food Agrie. 1966;17:182-8.

Ramdani D, Chaudhry AS, Seal CJ. Chemical composition, plant secondary metabolites, and minerals of green and black teas and the effect of different tea-to-water ratios during their extraction on the composition of their spent leaves as potential additives for ruminants. J Agric Food Chem. 2013;61:4961-7.

Kumaraguru Vasagam K, Rajkumar M. Beneficial influences of germination and subsequent autoclaving of grain legumes on proximate composition, antinutritional factors and apparent digestibility in black tiger shrimp, Penaeus monodon Fabricius. Aquaculture Nutrit. 2011;17:e188-e95.

Sahoo A, Singh B, Bhat T. Effect of tannins on in vitro ruminal protein degradability of various tree forages. Livest Res Rural Dev. 2010;22.

Shahidi F, Naczk M. Contribution of phenolic compounds to flavor and color characteristics of foods. Boca Raton: CRC Press; 2004. 443-63 p.

AOAC. Official Methods of Analysis of the Association of Official Analytical Chemists. 16th ed. USA: Virgin; 1995.

Shastry M, John E. Biochemical changes and in‐vitro protein digestibility of the endosperm of germinating dolichos lablab. J Sci Food Agri. 1991;55:529-38.

ELKhier MKS, Abd-Al Raheem AA. Effect of fermentation period on the chemical composition, in vitro protein digestibility and tannin content in two Sorghum cultivars (Dabar and Tabat) in Sudan. J App Biosci. 2011;39:2602-6.

Payne PI. Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Ann Rev Plant Physiol. 1987;38:141-53.

Eltayeb A, Ali AO, Haron R. The chemical composition of Pigeon pea (Cajanus cajan) seed and functional properties of protein isolate. Pak J Nutr. 2010;9:1069-73.

Olawuni I, Ojukwu M, Eboh B. Comparative study on physio-chemical properties of pigeon pea (Cajanus cajan) flour and protein isolate. Int J Agri Food Sci. 2012;2:121-6.

Mottram DS. Flavour formation in meat and meat products: a review. Food Chem. 1998;62:415-24.

Spanier A, Miller J, editors. Role of proteins and peptides in meat flavor. ACS symposium series (USA); 1993.

Li Y, Dong Y. Preoperative music intervention for patients undergoing cesarean delivery. Int J Gynaecol Obstet. 2012;119:81-3.

Murwan K, Murwa A. Chemical composition, minerals, protein fractionation, and anti-nutrition factors in leaf of Hargel plant (Solenostemma argel). Eur J Scient Res. 2010;43:430-4.

Deshpande S, Sathe S, Salunkhe D, Cornforth DP. Effects of dehulling on phytic acid, polyphenols, and enzyme inhibitors of dry beans (Phaseolus vulgaris L.). J Food Sci. 1982;47:1846-50.

Yagoub A. A biophysical study on Roselle (Hibiscus sabdariffal.) seeds total proteins of the traditionally fermented food. Khartoum: University of Khartoum; 2003.

Bamishaiye E, Olayemi F, Awagu E, Bamshaiye O. Proximate and phytochemical composition of Moringa oleifera leaves at three stages of maturation. Advance Journal of Food Science and Technology. 2011.




DOI: http://dx.doi.org/10.20286/nova-jmbs-030441

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DOI Prefix: 10.20286